Genetic Engineering/Modification

This lesson covers:

  1. What 'genetic engineering' is & why it's done
  2. What 'gene therapy' is
  3. The pros and cons of genetic engineering 
  4. The steps of genetic engineering 

What is genetic engineering?

The changing of the gene pool by only breeding together individuals with the most favourable traits 

The deletion of a gene from an organism

The transfer of a piece of DNA from one organism to another organism

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Illustration of a DNA double helix with coloured bases representing genetic material.

What is another name for genetic engineering? 

Genetic mutation 

Genetic modification 

Genetic changing 

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Genetic engineering can only be done between two organisms of the same species. 

True

False 

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Sheep have been genetically engineered to produce drugs in their .

milk

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Which of the following has been genetically engineered to produce human insulin?

Humans 

Bacteria 

Viruses 

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Give three traits that could have been altered by genetic engineering in crops. 

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What are the drawbacks of genetic engineering? 

(Select all that apply)

We can create crops with higher levels of vitamins 

If the crops breed with wild plants they could spread the new genes and affect the gene pool

There is a small risk that genetically modified food could negatively affect our health 

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Steps of genetic engineering for human insulin


plasmid / bacteria / recombinant / restriction / ligase


  1. Isolate the insulin gene from human DNA using enzymes, forming sticky ends.
  2. Use the same restriction enzymes to cut open a bacterial , forming complementary sticky ends. 
  3. Insert the the insulin gene into the plasmid using the enzyme DNA , to form a plasmid.
  4. Insert the recombinant plasmid into the host
  5. Let the bacteria divide and then produce the insulin protein. 
restriction
plasmid
ligase
recombinant
bacteria

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Illustration of a bacterium showing small circles of DNA known as plasmids.

What is the name of the small circles of DNA that are genetically modified in bacteria?

Plasmids

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Which two enzymes are used during the process of genetic engineering? 

Restriction enzymes 

DNA ligase 

DNA helicase

Protease enzymes 

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