Factors Affecting Enzyme Action

This lesson covers:

  1. How temperature affects the rate of enzyme controlled reactions 
  2. How pH affects the rate of enzyme controlled reactions
Illustration of an enzyme character with a flame on its head and sitting on ice, representing optimum temperature for enzyme action.

What does the term 'optimum' mean for enzyme temperature?


The highest temperature before denaturing occurs

The temperature at which enzyme activity is highest

The lowest temperature at which the enzyme works

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What is pH?

A measure of pressure 

A measure of salt concentation

A measure of acidity

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What affect does extremely high pH have on enzymes?

Inactivates them

Denatures them

Destroys them

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Which of these statements about enzymes is true?

They can be reused

They all have an optimum pH of 7

Temperature has no effect on their activity

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Which two of the following statements about enzymes are true?

Enzymes are reactants in chemical reactions

Enzymes are metals

Enzymes break down food during digestion

The shape of an enzyme is crucial for its activity

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Graph showing the rate of reaction increasing with temperature up to 37°C, then decreasing.

The graph above shows that as temperature increases from 0°C to 37°C, the rate of reaction ________.

Decreases

Stays the same

Increases

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Reading from the graph, what's the optimum temperature for enzyme activity?

37

°C

°C

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Once the temperature rises above 37°C, enzyme activity begins to .

decrease

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Graph showing the rate of reaction for pepsin and amylase at different pH levels.

Pepsin is found in the stomach, amylase is found elsewhere in the body.

Using the graph above, what is the optimum pH for pepsin?

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Explain how increasing the temperature can cause an enzyme to denature.

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