Welcome to the Quiz!

This quiz contains 11 questions from a mix of 1 subtopics.

What is the primary structure of a protein?

the folding patterns like α-helices and β-pleated sheets

the overall 3D shape of the protein

the sequence of amino acids in the polypeptide chain

the interactions between R groups

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What happens to proteins when they undergo denaturation?

they gain a new function due to a change in shape

they lose their function due to a permanent change in shape

they become more stable and resistant to temperature changes

they break down into individual amino acids

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What is the purpose of developing agents like ninhydrin or ultraviolet light in thin layer chromatography?

to identify the amino acids

to make the amino acid spots visible

to separate the amino acids

to calculate the Rf values

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What type of bond forms between the amine group of one amino acid and the carboxyl group of another during protein synthesis?

ionic bond

hydrogen bond

peptide bond

disulfide bridge

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What molecule is released during the formation of a peptide bond between two amino acids?

ammonia

oxygen

water

carbon dioxide

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Which of the following is true about the tertiary structure of a protein?

it is formed by hydrogen bonds between peptide bonds

it refers to the overall 3D shape of the protein

it is the sequence of amino acids in the polypeptide chain

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What are the two main types of bonds that help maintain the secondary and tertiary structures of proteins?

peptide bonds and ionic bonds

disulfide bridges and covalent bonds

covalent bonds and hydrogen bonds

hydrogen bonds and disulfide bridges

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In thin layer chromatography, what determines the speed at which amino acids travel up the TLC plate?

their solubility in the solvent

their size

their charge

the strength of the developing agent

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What type of reaction occurs when polypeptide chains are broken back down into amino acids using hot aqueous acid?

hydrolysis

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What type of reaction is involved in the formation of peptide bonds?

reduction

oxidation

condensation

hydrolysis

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What is the equation used to calculate the Rf value in thin layer chromatography?

Rf =distance moved by solvent frontdistance moved by spot
Rf = distance moved by solvent front× distance moved by spot
Rf =distance moved by spotdistance moved by solvent front
Rf = distance moved by solvent front+ distance moved by spot

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