Welcome to the Quiz!
This quiz contains 11 questions from a mix of 1 subtopics.
Which of the following is true about batch fermentation?
microbes are grown in individual batches, each followed by emptying and cleaning of the vessel
the culture grows indefinitely without the need for cleaning
fresh medium is continuously supplied while products are removed
it avoids the issue of nutrient depletion and waste accumulation
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In which phase of batch culture growth do cells show slow initial growth as they adapt and produce essential enzymes?
stationary phase
log phase
death phase
lag phase
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What is the purpose of preparing a control agar dish with no bacteria when culturing microbes in the laboratory?
to ensure that the agar is not contaminated
to investigate the effects of antimicrobial substances
to quantify growth differences using a spectrophotometer
to compare the growth of different microbial species
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What is the main advantage of continuous fermentation over batch fermentation?
it requires less frequent cleaning and sterilisation of the vessel
it follows a standard growth pattern over time
it allows for the study of factors influencing microbial growth
it avoids the issues of nutrient depletion and waste accumulation
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What is the purpose of steam sterilisation in bioreactors?
to prevent unwanted microbial contamination and competition
to provide oxygen for aerobically respiring microbes
to adjust the pH of the culture medium
to ensure the even distribution of nutrients
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Which of the following is true about the stationary phase in batch culture growth?
cells show slow initial growth as they adapt and produce essential enzymes
growth plateaus as nutrients diminish and waste accumulates
rapid doubling of cell numbers occurs under ideal conditions
cell death exceeds cell growth due to resource limitation and build up of toxins
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What is the purpose of using a sterile wire loop or pipette when transferring microbes from a starter culture to an agar plate?
to prevent contamination of the starter culture
to maintain the desired temperature for microbial growth
to ensure even distribution of the sample across the agar surface
to adjust the pH of the agar medium
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Which of the following is true about the log phase in batch culture growth?
growth plateaus as nutrients diminish and waste accumulates
cells show slow initial growth as they adapt and produce essential enzymes
rapid doubling of cell numbers occurs under ideal conditions
cell death exceeds cell growth due to resource limitation and build up of toxins
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Which of the following is NOT a step in growing microbes on agar plates?
transferring microbes from a starter culture to the agar plate using a sterile wire loop or pipette
sterilising all equipment before use
sealing the plates completely with tape
incubating the plates upside down under the required conditions
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Which of the following is true about continuous fermentation?
it requires frequent cleaning and sterilisation of the vessel between batches
it involves growing microbes in individual batches, each followed by emptying and cleaning of the vessel
it involves continuously supplying fresh medium and removing products while the culture grows indefinitely
it follows a standard growth pattern over time, including lag, log, stationary, and death phases
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Describe the key steps involved in culturing microbes on agar plates in the laboratory.
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