Welcome to the Quiz!
This quiz contains 11 questions from a mix of 1 subtopics.
What is the process of deliberately manipulating genetic material to modify an organism's characteristics called?
replication
cloning
genetic engineering
selective breeding
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Which of the following is NOT a key stage in gene transfer?
identifying and isolating the desired gene
inserting the gene into a vector
removing the gene from the transgenic organism
making multiple copies of the gene using PCR
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What enzyme is used to produce complementary DNA (cDNA) from mRNA templates?
RNA polymerase
DNA polymerase
reverse transcriptase
restriction endonuclease
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What are the short overhangs of unpaired bases on cut DNA called?
complementary ends
sticky ends
palindromic sequences
blunt ends
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Which of the following is true about restriction enzymes?
they are used to reverse transcribe mRNA into cDNA
they are used to synthesise custom DNA sequences
they recognise and cut DNA at specific palindromic nucleotide sequences
they are used to amplify DNA fragments
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What are palindromic sequences in DNA?
sequences that are recognised by DNA polymerase
sequences that read the same in opposite directions on antiparallel strands
sequences that code for the same amino acid
sequences that are complementary to mRNA
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What is the primary role of vectors in gene transfer?
to make multiple copies of the gene
to express the transferred gene and produce the encoded protein
to identify and isolate the desired gene
to deliver the gene into cells
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Which of the following is true about transgenic organisms?
they contain DNA fragments from another organism
they are created through selective breeding
they are always genetically identical to the donor organism
they cannot express transferred genes due to the specificity of the genetic code
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Describe the key stages in the process of gene transfer.
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What is the primary advantage of using mRNA to produce cDNA compared to extracting DNA directly from cells?
mRNA is more stable than DNA
mRNA can be directly inserted into vectors
mRNA is easier to extract from cells than DNA
mRNA contains more copies of the desired gene than DNA
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What is the role of DNA polymerase in the production of cDNA?
it reverse transcribes mRNA into cDNA
it forms the complementary strand to cDNA
it inserts the cDNA into a vector
it amplifies the cDNA
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