Welcome to the Quiz!
This quiz contains 10 questions from a mix of 1 subtopics.
What is gel electrophoresis used for?
separating molecules based on size
separating molecules based on charge
separating molecules based on colour
separating molecules based on shape
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Which of the following molecules can be separated using gel electrophoresis?
DNA
RNA
proteins
all of the above
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What is the purpose of submerging the gel in buffer solution during electrophoresis?
to make the samples visible
to prevent the gel from drying out
to apply an electrical current
to maintain a stable and appropriate pH
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Which electrode should the wells be positioned near when setting up the gel?
positive electrode (anode)
negative electrode (cathode)
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During electrophoresis, in which direction do DNA molecules move?
they do not move
randomly in all directions
towards the negative electrode (cathode)
towards the positive electrode (anode)
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Which fragments of DNA travel faster during electrophoresis?
all fragments travel at the same speed
larger fragments
the speed is not dependent on size
smaller fragments
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What does NOT affect the rate at which DNA fragments move through the gel?
the pore size of the gel
the voltage applied
the light intensity
the size of the fragments
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What is Southern blotting used for?
transferring single-stranded DNA from the gel to a membrane
staining the DNA fragments
measuring the size of the DNA fragments
applying voltage to the gel
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What is the purpose of treating proteins with a denaturing agent before electrophoresis?
to prevent them from moving
to make them visible
to standardise their charge
to make them move faster
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Describe how gel electrophoresis separates DNA fragments and how the results can be visualised.
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