Welcome to the Quiz!
This quiz contains 10 questions from a mix of 1 subtopics.
What is the primary function of PCR?
to rapidly replicate specific DNA fragments
to add primers to DNA
to separate DNA strands
to genetically modify DNA strands
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Which of the following components is NOT required for PCR?
DNA polymerase
DNA fragments
primers
DNA ligase
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What type of DNA polymerase is used in PCR?
one that is inactive at low temperatures
one that is resistant to high temperatures
one that is sensitive to high temperatures
one that is inactive at high temperatures
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What is the purpose of the thermocycler in PCR?
to precisely heat and cool the PCR mixture
to attach primers to the DNA
to add free nucleotides to the reaction
to separate the DNA strands
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What happens during the separation (denaturation) stage of PCR?
DNA polymerase adds nucleotides
hydrogen bonds between DNA strands break
new DNA strands are synthesised
primers attach to the DNA
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What happens during the DNA synthesis (extension) stage of PCR?
the reaction mixture is heated to 95°C
DNA strands separate
primers attach to the DNA
DNA polymerase adds nucleotides to form a complete copy
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Which of the following is NOT an advantage of PCR?
low DNA needs
rapid speed
precision
uses living cells
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What is the purpose of primers in PCR?
to synthesise new DNA strands
to separate the DNA strands
to add nucleotides to the DNA
to attach to the start and end of the DNA fragment to be copied
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What is the purpose of heating the DNA to 95°C in PCR?
to separate the DNA strands
to cool the reaction mixture
to synthesise new DNA strands
to attach primers to the DNA
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Describe the 3 key stages of PCR and state the temperature at which each stage occurs.
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