Welcome to the Quiz!
This quiz contains 12 questions from a mix of 1 subtopics.
What is the purpose of using restriction enzymes in the formation of recombinant DNA?
to transfer the recombinant DNA into host cells
to create sticky ends on the vector and DNA fragment
to amplify the target DNA fragment
to join the vector and DNA fragment together
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Which enzyme forms phosphodiester bonds between the sugar and phosphate groups on the two strands of DNA, joining the sticky ends of the vector and DNA fragment together?
RNA polymerase
DNA ligase
restriction enzyme
DNA polymerase
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What is the combined DNA molecule formed by joining a vector and a DNA fragment called?
bacteriophage DNA
plasmid DNA
complementary DNA
recombinant DNA
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Which of the following are vectors that can be used to introduce recombinant DNA into host cells?
neither plasmids nor bacteriophages
both plasmids and bacteriophages
plasmids
bacteriophages
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What are plasmid vectors?
small, circular DNA molecules found in bacteria
viruses that infect bacteria
enzymes that cut DNA at specific sites
large, linear DNA molecules found in bacteria
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What happens when bacteriophages infect bacterial cells?
they replicate independently of the host bacterial cells
they inject their DNA into the host bacterial cells
they extract DNA from the host bacterial cells
they destroy the host bacterial cells
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What is the purpose of marker genes in the formation of recombinant DNA?
to transfer the recombinant DNA into host cells
to join the vector and DNA fragment together
to indicate which host cells have been successfully transformed
to amplify the target DNA fragment
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Which of the following is an example of a marker gene that can be used to identify transformed host cells?
a gene for antibiotic resistance
a gene that is visible under UV light, like green fluorescent protein (GFP)
a gene coding for an enzyme that alters the colour of a specific substrate
all of the above
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What happens when a marker gene is inserted within the GFP gene in a vector?
it inhibits fluorescence if successfully incorporated
it causes the host cell to produce a different color
it has no effect on fluorescence
it enhances fluorescence if successfully incorporated
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Describe the key steps involved in inserting a DNA fragment into a vector to form recombinant DNA.
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is when an electrical current makes bacterial membranes more porous, facilitating the entry of plasmids into bacterial cells.
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Which of the following treatments can enhance the uptake of plasmids with recombinant DNA by host cells?
(Select all that apply)
application of chloride ions
electroporation
temperature shifts
keeping a constant temperature
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