Welcome to the Quiz!
This quiz contains 10 questions from a mix of 1 subtopics.
What is the primary function of PCR?
to separate DNA strands
to add primers to DNA
to genetically modify DNA strands
to rapidly replicate specific DNA fragments
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Which of the following components is NOT required for PCR?
DNA ligase
primers
DNA polymerase
DNA fragments
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What type of DNA polymerase is used in PCR?
one that is inactive at low temperatures
one that is sensitive to high temperatures
one that is resistant to high temperatures
one that is inactive at high temperatures
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What is the purpose of the thermocycler in PCR?
to precisely heat and cool the PCR mixture
to add free nucleotides to the reaction
to attach primers to the DNA
to separate the DNA strands
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What happens during the separation (denaturation) stage of PCR?
DNA polymerase adds nucleotides
new DNA strands are synthesised
primers attach to the DNA
hydrogen bonds between DNA strands break
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What happens during the DNA synthesis (extension) stage of PCR?
the reaction mixture is heated to 95°C
DNA strands separate
primers attach to the DNA
DNA polymerase adds nucleotides to form a complete copy
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Which of the following is NOT an advantage of PCR?
uses living cells
low DNA needs
rapid speed
precision
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What is the purpose of primers in PCR?
to add nucleotides to the DNA
to synthesise new DNA strands
to separate the DNA strands
to attach to the start and end of the DNA fragment to be copied
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What is the purpose of heating the DNA to 95°C in PCR?
to attach primers to the DNA
to cool the reaction mixture
to synthesise new DNA strands
to separate the DNA strands
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Describe the 3 key stages of PCR and state the temperature at which each stage occurs.
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