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This quiz contains 10 questions from a mix of 1 subtopics.

Give three properties of the solution used in cell fractionation to prevent damage to organelles.

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During cell fractionation, why must the solution be homogenised?

to separate the organelles

to break open the cells 

to prevent enzymes from denaturing

to remove cell debris

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After filtration, which speed should the solution be spun at?

low speed

high speed

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What is the order of organelles from heaviest to lightest?

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In cell fractionation, why must the tissue sample be added to an ice-cold solution?

to prevent osmosis

to denature the enzymes

to reduce enzyme activity

to maintain the pH

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In cell fractionation, the tissue sample must be added to an isotonic solution to prevent damage by .

osmosis

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In cell fractionation, why must the tissue sample be added to a buffer solution?

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Which step in cell fractionation removes debris?

filtration

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The step in cell fractionation that involves separating the cell organelles is known as .

ultracentrifugation

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Which organelle collects in the first pellet during ultracentrifugation?

nuclei

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