Welcome to the Quiz!
This quiz contains 13 questions from a mix of 1 subtopics.
What is the purpose of using restriction enzymes in the formation of recombinant DNA?
to join the vector and DNA fragment together
to create sticky ends on the vector and DNA fragment
to transfer the recombinant DNA into host cells
to amplify the target DNA fragment
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Which enzyme forms phosphodiester bonds between the sugar and phosphate groups on the two strands of DNA, joining the sticky ends of the vector and DNA fragment together?
DNA polymerase
DNA ligase
restriction enzyme
RNA polymerase
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What is the combined DNA molecule formed by joining a vector and a DNA fragment called?
complementary DNA
bacteriophage DNA
recombinant DNA
plasmid DNA
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Which of the following are vectors that can be used to introduce recombinant DNA into host cells?
both plasmids and bacteriophages
bacteriophages
neither plasmids nor bacteriophages
plasmids
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What are plasmid vectors?
large, linear DNA molecules found in bacteria
small, circular DNA molecules found in bacteria
viruses that infect bacteria
enzymes that cut DNA at specific sites
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What happens when bacteriophages infect bacterial cells?
they inject their DNA into the host bacterial cells
they extract DNA from the host bacterial cells
they destroy the host bacterial cells
they replicate independently of the host bacterial cells
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What is the purpose of marker genes in the formation of recombinant DNA?
to transfer the recombinant DNA into host cells
to indicate which host cells have been successfully transformed
to amplify the target DNA fragment
to join the vector and DNA fragment together
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Which of the following is an example of a marker gene that can be used to identify transformed host cells?
a gene for antibiotic resistance
a gene that is visible under UV light, like green fluorescent protein (GFP)
a gene coding for an enzyme that alters the colour of a specific substrate
all of the above
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What happens when a marker gene is inserted within the GFP gene in a vector?
it inhibits fluorescence if successfully incorporated
it enhances fluorescence if successfully incorporated
it causes the host cell to produce a different color
it has no effect on fluorescence
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Describe the key steps involved in inserting a DNA fragment into a vector to form recombinant DNA.
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What are the control elements that a vector needs for the protein coded by an inserted DNA fragment to be produced?
neither promoter nor terminator regions
terminator regions only
promoter regions only
both promoter and terminator regions
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What is the function of promoter regions in a vector?
to join the vector and DNA fragment together
to provide a binding area for RNA polymerase and signal where transcription should start
to indicate where transcription should end and release RNA polymerase
to transfer the recombinant DNA into host cells
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What is the function of terminator regions in a vector?
to indicate where transcription should end and release RNA polymerase
to transfer the recombinant DNA into host cells
to provide a binding area for RNA polymerase and signal where transcription should start
to join the vector and DNA fragment together
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