What is used as the solvent in the chromatography practical?

Water.

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Where should the origin line be drawn on the chromatography paper?

2 cm from the bottom short edge of the paper.

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What is the purpose of the solvent front line?

To mark how far the solvent travelled up the paper.

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What measurements are needed to calculate Rf values?

  1. Distance between start line and solvent front line (solvent distance).
  2. Distance between the start line and the middle of each colour spot.

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What does a higher Rf value mean?

The higher the Rf value, the further up the chromatography paper the substance travelled.

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Why hang up the chromatography paper to dry before calculating Rf values?

To prevent the spots from continuing to move or diffuse, allowing accurate distance measurements to calculate Rf.

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