How does a laser scanning confocal microscope work?
A laser scanning confocal microscope uses a laser beam to scan a specimen that has been tagged with fluorescent dye.
The dyed components give off light which is focused through a pinhole and onto a detector.
A computer connected to the detector generates an image.
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What does a pinhole do in a laser scanning confocal microscope?
The pinhole blocks any out-of-focus light to produce clearer images.
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Compare the resolution of a laser scanning confocal microscope with light and electron microscopes.
Laser scanning confocal microscopes have a better resolution than light microscopes, but a worse resolution than electron microscopes.
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Give two uses of a laser scanning confocal microscope.
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What are specimens tagged with in laser scanning confocal microscopy, and why?
Specimens are tagged with a fluorescent dye, which glows when light is shone on them during laser scanning confocal microscopy.
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