What is micropropagation?

Micropropagation is a technique to produce many identical plant clones from a single parent plant using tissue culture.

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What makes meristem cells useful for micropropagation?

Meristem cells are totipotent, meaning they can differentiate into all plant cell types.

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What is a tissue culture in micropropagation?

A tissue culture involves growing plant tissues in a sterile medium with hormones like auxins and cytokinins to stimulate cell division and growth.

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What is an explant?

An explant is a small tissue sample taken from a plant to start the micropropagation process.

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What is a callus?

A callus is a mass of undifferentiated plant cells that forms from an explant.

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How are plantlets formed?

Plantlets are formed by transferring callus cells to a new medium that stimulates plantlet growth.


Plantlets can then be potted in compost to grow into individual plants.

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Why is sterilisation of a sample important in micropropagation?

Sterilisation reduces the risk of widespread infection and produces healthier crops.

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What are some of the key disadvantages of micropropagation?

  1. It is expensive and requires skilled technicians
  2. Explants and plantlets are vulnerable to infection
  3. All plants are genetically identical (monoculture) so are vulnerable to environmental changes


Plants in a monoculture are particularly vulnerable to being wiped out by a new disease, pest, or climate change.

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What are some of the key advantages of micropropagation?

  1. Produces large numbers of plants quickly
  2. Produces plants that are genetically identical so are known to produce high yields
  3. Culturing usually produces disease-free plants
  4. Can produce seedless plants
  5. Can produce plants that are hard to grow from seeds
  6. Enables the rapid increase of the numbers of rare or endangered plants

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