What is photoactivation in the context of photosynthesis?

Photoactivation is the emission of an electron from a molecule as a result of the absorption of energy from light. 

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What are the main reactant(s) of the light-dependent reaction?

The main reactants of the light-dependent reaction are water (and light energy).

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What are the product(s) of the light-dependent reaction?

The products of the light-dependent reaction, specifically non-cyclic photophosphorylation, are ATP, reduced NADP, and oxygen.


Reduced NADP and oxygen are not produced in cyclic photophosphorylation.

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What are the key stages of the light-dependent reaction?

  1. Absorption of light by pigments
  2. Release of electrons from pigments
  3. Electron transfer along the electron transport chain
  4. Photolysis (splitting) of water to provide protons (H+) and electrons (e-, to replace those lost)
  5. Chemiosmosis (movement of protons through the thylakoid membrane)
  6. Production of ATP and reduced NADP

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What is the role of chemiosmosis in photosynthesis?

In photosynthesis, chemiosmosis is the diffusion of protons across the partially permeable thylakoid membrane, down their electrochemical gradient through ATP synthase channels.


The movement of the protons releases energy that is used to synthesise ATP.

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What is the role of the electron transport chain in the light-dependent reaction?

The electron transport chain transfers excited electrons from photosynthetic pigments to electron carriers.


This transfer releases energy, which is used to pump protons across the thylakoid membrane, creating a proton gradient that drives ATP synthesis.

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What is the equation for the photolysis of water, and how is the process useful in photosynthesis?

The equation for the photolysis of water is:

2H2O + light energy ➔ 4H+ + 4e- + O2


Photolysis produces electrons to replace those lost by pigments. It produces protons that help set up the proton gradient that drives ATP synthesis. The protons and electrons are also used to reduce NADP. Photolysis produces oxygen as a by-product.


NADP is reduced when it takes up protons produced during photolysis, carrying the electrons into the light-independent reaction.

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How is ATP produced in the light-dependent reaction, including reference to ATP synthase and NADP?

ATP synthase catalyses the production of ATP from ADP and Pi. This uses the energy from the flow of protons down their concentration gradient from the thylakoid space into the stroma through ATP synthase channels.


The protons are then taken up by NADP in the stroma and, along with electrons from the electron transport chain, produce reduced NADP for use in the light-independent reaction.

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Outline the process of non-cyclic photophosphorylation.

  1. Light absorption excites electrons at the reaction centres of PSI and photosystem II (PSII)
  2. The excited electrons emitted from reaction centres are passed along chains of electron carriers
  3. Energy is released, allowing protons to be pumped into the thylakoid space
  4. The electrons lost from PSII replace those lost from PSI
  5. Photolysis of water generates electrons (to replace those lost from PSII) and more protons in the thylakoid space
  6. Protons diffuse through ATP synthase into the stroma, and ATP is synthesised via chemiosmosis
  7. The electrons lost from PSI and protons pumped through ATP synthase are used to reduce NADP

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Outline the process of cyclic photophosphorylation.

  1. Light absorption excites electrons at the reaction centres of photosystem I (PSI)
  2. The excited electrons emitted from reaction centres and are passed along chains of electron carriers
  3. The electrons lose energy as they pass along the chain, which is used to actively move protons from the stroma, across the thylakoid membrane, into the thylakoid space
  4. The protons can the move back into the stroma down their concentration gradient through ATP synthase to generate ATP


NADP is not reduced in this process.

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