Why do electron microscopes have a better resolution than light microscopes?

Electron microscopes have a better resolution than light microscopes because electrons have a shorter wavelength than light.

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Name two types of electron microscope.

  1. Transmission electron microscope (TEM)
  2. Scanning electron microscope (SEM)

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Give three differences between light microscopes and electron microscopes.

  1. Electron microscopes have a better resolution
  2. Electron microscopes have a higher magnification
  3. Electron microscopes are more expensive 
  4. Electron microscopes produce black and white images, light microscopes produce colour images 
  5. Electron microscopes require more complex sample preparation 
  6. Electron microscopes only use dead specimens, light microscopes can use living specimens

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What is an artefact?

An artefact is a visible detail that isn't part of the specimen being observed.


For example, air bubbles or fingerprints.

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How do TEMs produce images?

TEMs transmit a beam of electrons through a specimen. More dense parts of the specimen absorb more electrons, they so appear darker in the image formed.

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Why must organisms be dead or non-living when using electron microscopes?

Organisms must be dead or non-living for electron microscopes because the specimen is viewed in a vacuum.

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Why must the specimen be thin when using a TEM?

The specimen must be thin when using a TEM to allow electrons to pass through.

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What is the magnification and resolution of a TEM?

The magnification of a typical TEM is x 500,000 and the resolution is 0.2 nm to 1.0 nm.

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How do scanning electron microscopes (SEMs) produce images?

SEMs scan a beam of electrons across the surface of a specimen. Reflected electrons are then used to form an image.

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Describe the difference between images produced by a TEM and an SEM.

A TEM produces 2D images at a better resolution, whereas an SEM produces 3D images at a worse resolution.

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Which type of electron microscope can be used on thicker specimens?

Scanning electron microscopes (SEMs) can be used on thicker specimens.

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What is the magnification and resolution of an SEM?

The magnification of a typical SEM is x 100,000 and the resolution is 1 nm to 20 nm.

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Give three similarities between TEMs and SEMs.

  1. Both are expensive
  2. Both involve complex sample preparation
  3. Both produce black and white images
  4. Both only view dead specimens 

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