What does enzyme affinity refer to?

The strength of the attraction between an enzyme and its substrate.

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What does a high enzyme affinity mean?

High affinity means there is a strong attraction between the enzyme and its substrate.

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What does a low enzyme affinity mean?

Low affinity means there is a weak attraction between the enzyme and its substrate.

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What is Vmax?

Vmax is the maximum rate of an enzyme-catalyzed reaction

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What is the saturation point in an enzyme-catalysed reaction?

The point where all active sites of an enzyme are occupied by a substrate.

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What is Km?

Km is the substrate concentration at which half the enzyme's active sites are bound to substrates.

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How can Km be calculated from a graph?

  1. Calculate Vmax
  2. Divide Vmax by 2
  3. Read the substrate concentration from the x axis at  12\frac{1}{2}Vmax

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What does a lower Km value indicate?

A lower Km value indicates a higher affinity of an enzyme for its substrate.

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What does a higher Km value indicate?

A higher Km value indicates a lower affinity of an enzyme for its substrate. 

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