What is the role of enzymes in human digestion?

Enzymes produced by the digestive system hydrolyse large insoluble food molecules into smaller soluble molecules that can be absorbed.

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Name the main organs of the human digestive system in the order that food travels through them.

  1. Mouth
  2. Oesophagus
  3. Stomach
  4. Small intestine
  5. Large intestine
  6. Rectum
  7. Anus

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What enzymes are produced by the pancreas and what do they digest?

The pancreas produces:

  1. Proteases - digest proteins
  2. Lipase - digests lipids
  3. Amylase - digests carbohydrates like starch

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What is the difference between chemical and mechanical digestion?

  1. Chemical digestion is the enzymatic hydrolysis of molecules into smaller absorbable units
  2. Mechanical digestion is the physical breakdown of food, for instance by chewing

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Describe the breakdown of starch by enzymes in digestion, including where the enzymes are made and where they act.

  1. Salivary amylase hydrolyses starch to maltose - this enzyme is produced by the salivary glands and acts in the mouth
  2. Pancreatic amylase continues to hydrolyse starch to maltose - this enzyme is produced in the pancreas and acts in the small intestine

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Describe the breakdown of maltose by enzymes in digestion, including where the enzymes are made and where they act.

Maltase hydrolyses maltose to α-glucose.


Maltase is produced by the ileum's epithelial cells and hydrolysis occurs at the membrane of the ileum's epithelial cells.


Maltase is therefore referred to as a membrane-bound disaccharidase.

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Describe the breakdown of protein by enzymes in digestion, including where the enzymes are made and where they act.

Proteases (peptidases) hydrolyse proteins into smaller peptides and then into amino acids.


Proteases that are produced by the stomach act in the stomach. Peptidases that are produced by the pancreas and the ileum's epithelial cells act in the small intestine.

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Compare endopeptidases, exopeptidases and dipeptidases.

  1. Endopeptidases hydrolyse internal peptide bonds to produce smaller polypeptides
  2. Exopeptidases hydrolyse terminal peptide bonds of polypeptides
  3. Dipeptidases hydrolyse bonds between two amino acids in a dipeptide


Dipeptidases are membrane-bound enzymes.

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Describe the breakdown of lipids by enzymes in digestion, including where the enzymes are made and where they act.

Lipases hydrolyse lipids into fatty acids and monoglycerides. 


Lipases are produced by the pancreas and act in the small intestine.

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What is the role of emulsification in lipid digestion?

Emulsification of lipids by bile salts increases the surface area of the lipids by splitting them into droplets called micelles. This allows faster hydrolysis of lipids by lipase enzymes.


Bile salts are produced by the liver and stored in the gallbladder before being secreted into the small intestine.

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