What is magnification?

Magnification is how many times larger an image is than the actual object.

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What is resolution?

Resolution is the ability to distinguish between two separate points.

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Convert 20 mm into μm.

20 mm is 20,000 μm

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Convert 340 nm into μm.

340 nm is 0.34 μm

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What is the equation for magnification?

magnification = actual sizeimage size​

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Rearrange the magnification equation to make actual size the subject.

actual size = magnificationimage size​

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Rearrange the magnification equation to make image size the subject.

image size = actual size × magnification

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What is an eyepiece graticule?

An eyepiece graticule is a small scale placed within the eyepiece.

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What is a stage micrometer?

A stage micrometer is a glass slide with a scale measured in µm.

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Describe the steps to calibrate an eyepiece graticule.

  1. Fix a stage micrometer onto the stage. 
  2. Look through the eyepiece to line up the micrometer and the graticule.
  3. Count the number of graticule divisions that fit into one micrometer division. 
  4. Use the formula below to calculate the size of each graticule division.


Graticule division =Number of graticule divisionsSize of one micrometer division​

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