What stain is used on onion skin slides?

Iodine solution

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What is the purpose of methylene blue when making cheek cell slides?

It selectively binds to DNA, resulting in a darker blue stain of cell nuclei. This makes it easier to identify cells under the microscope.

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What is the order of steps when focusing under the microscope?

  1. Select the lowest power objective lens.
  2. Use the coarse adjustment knob to lower the lens close to the slide.
  3. Look through the eyepiece and use the coarse adjustment knob to bring cells into focus.
  4. Refine focus with the fine adjustment knob.

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How do you calculate total magnification when drawing observed cells?

Total magnification = lens magnification x Objective lens magnification

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What are the guidelines for good scientific drawings?

  1. Label with sample name and magnification
  2. Use pencil
  3. Neat outlines, no sketchy lines
  4. No shading or colour
  5. Clear labels with ruler lines
  6. Clean, no smudges

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What does a microscope coarse adjustment knob do?

Moves the objective lens towards or away from the slide more rapidly and in larger increments.

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What does the microscope fine adjustment knob do?

Makes precise and subtle changes to the focus for an optimised, clear image.

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