What is used to quantitatively assess the effect of antiseptics/antibiotics on bacteria?
Agar diffusion tests - applying agents to agar plates with bacteria and measuring zones of inhibition.
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Why is it important to ensure your hands and work area are clean before starting the microbiology practical?
To prevent contamination of the agar plate with unwanted microbes that could interfere with results.
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What is the purpose of dividing the agar plate into sections?
To test different antiseptics in different areas of the plate.
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Why should the agar plate not be completely sealed with tape?
To prevent anaerobic conditions that could inhibit bacterial growth or encourage harmful bacterial growth.
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How can the effect of different antiseptics be compared?
By comparing the diameters of the zones of inhibition - larger zones indicate more effective bacterial inhibition.
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